Primers, probes and plasmid development for specific PCR detection of genetically modified common bean (Phaseolus vulgaris) Embrapa 5.1
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چکیده
منابع مشابه
Development of Phaseolus vulgaris endogenous reference and Embrapa 5.1 event-specific assays for quantification of Embrapa 5.1 GM common bean using real time PCR
Background Brazil is the second largest producer of genetically modified (GM) crops with 37 GM crop varieties approved by CTNBio for commercialization. One of these varieties is the Embrapa 5.1 common bean, resistant to the bean golden mosaic virus (BGMV). The Embrapa 5.1 event was developed using the RNAi concept to induce the silencing of the AC1 viral gene [1]. Labelling of food products con...
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Low-stringency single specific primer polymerase chain reaction (LSSP)-PCR was assessed for its suitability in detecting the genotoxic effect of paranitrophenol (PNP) in the dwarf bean (Phaseolus vulgaris) exposed to different concentrations of PNP. DNA was extracted from both PNP-treated and non-treated shoots that was amplified by specific PCR, using universal primers of maturase K chloroplas...
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Bean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%)....
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Next generation sequence data provides valuable information and tools for genetic and genomic research and offers new insights useful for marker development. This data is useful for the design of accurate and user-friendly molecular tools. Common bean (Phaseolus vulgaris L.) is a diverse crop in which separate domestication events happened in each gene pool followed by race and market class div...
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